Regulatory

Part:BBa_I728456:Design

Designed by: Bernice Huang   Group: iGEM07_MIT   (2007-10-23)


MerRT: Mercury-Inducible Promoter+RBS (MerR + part of MerT)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 421
    Illegal SapI site found at 590


Design Notes

The MerR protein sequence is transcribed in the reverse direction from the rest of the operon.


Source

This part was PCR'ed off of plasmid pT040 from the paper "Metalloregulatory DNA-Binding Protein Encoded by the merR Gene: Isolation and Characterization" by Thomas O'Halloran and Christopher Walsh. The plasmid pT040 contains part of Tn501, Genbank accession: Z00027.

References